What is the mechanism for the three-part differential found in older Coulter machines or those designed for smaller laboratories?
a. Volume, conductivity, scatter
b. Multiangle polarized scatter separation
c. Peroxidase staining and light scatter
d. Impedance after cytoplasm shrinkage
ANS: D
Older Coulter instruments use a special white blood cell (WBC) lysing agent that causes differential shrinkage of white cells, allowing the different white cells to be counted and sized based on their impedance.
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