Which of the following is important in processing a urine specimen for microscopic examination of sediment? 

A. Fill one test tube with 2 mL of water and the other test tube with 2 mL of urine
B. Balance the centrifuge by placing the test tubes upside down
C. Set the centrifuge for 20 minutes
D. After the centrifuge stops, discard most of the supernatant from the tube
E. Avoid using a coverslip on the specimen slide

D. After the centrifuge stops, discard most of the supernatant from the tube
After the centrifuge stops, discard most of the supernatant and mix the remaining few drops with the sediment by gently tapping the tube.