What principle does the Sysmex SE-9000/9500 use to analyze and separate white cell populations?
a. Electronic impedance
b. Optical light scatter
c. Volume, conductivity, and scatter
d. Low-frequency direct current and high-frequency current
ANS: D
The Sysmex 9000 analyzes WBCs by low-frequency direct current and high-frequency current. A scattergram of radiofrequency detection signals (y-axis) versus direct-current detection signals (x-axis) is plotted and yields separation of the white cells through the application of floating discriminators.
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