In the center square on one side of a hemocytometer, 100 platelets were found; 200 were found in the center square on the other side. Select the best course of action
a. Report the count as 150 ´ 109/L.
b. Verify the count by estimating the platelet number on the blood film.
c. Recount the hemocytometer on a phase microscope.
d. Repeat the procedure—the two counts are not close enough.
ANS: D
The cell counts on the two opposite sides of the hemocytometers must agree within 10% of each other. Thus if the count is 100 on one side, then the other side must be no higher than 100 + (0.1 ´ 100) = 110. Because 200 exceeds this, the counts do not agree—an error has been made. The dilutions must be repeated and then the new dilutions counted.
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