Name disorders in which the following should be included in the workup of a hematologic disorder. Explain why they are used
a. Flow cytometry
b. Cytogenetics
c. Molecular genetics
d. Cytochemical staining
What will be an ideal response?
Answer:
Flow cytometry: malignant lymphomas and leukemias; evaluates for minimal residual disease after chemotherapy
Cytogenetics: acute leukemias, malignant lymphomas, MDS, and MPDs; helps determine diagnosis and prognosis. Also can detect relapse.
Molecular genetics: hematolymphoid malignancies; can detect minimal residual disease
Cytochemical staining: subsets of acute leukemia; differential diagnosis of acute and chronic leukemias, such as ALL, AML, CML, CLL, and HCL.
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