Standard bicarbonate is calculated by use of which of the following?

a. Hender-son-Hasselbalch equation c. Lambert-Beer law equation
b. Two-point calibra-tion method d. Levy-Jennings chart

ANS: A
HCO3– is derived from measurements of pH and PaO2 by using the Henderson-Hasselbalch equa-tion. A two-point calibration is a quality assurance test that is performed at least three times daily for the measuring electrodes. The Lambert-Beer law is applied in oximetry to determine the con-centration of hemoglobin. A Levy-Jennings chart is used to record quality control data for a blood gas machine.

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