Which of the following is a morphologically identifiable precursor cell using Wright stain?
a. Promyelocyte
b. Colony-forming unit–erythrocyte (CFU-E)
c. Colony-stimulating factor–granulocyte-monocyte (CSF-GM)
d. HSC
ANS: A
Only the promyelocyte, which is an early granulocyte (neutrophil), can be identified using a Wright stain. None of the stem cells (CFU-E, CSF-GM, HSC) can be identified by morphology. The stem cells are functionally defined by their behavior in vitro using specific colony culture techniques. These require special growth media and specific cytokine (or cytokines). They can be identified using immunophenotypic techniques combined with flow cytometry.
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Fill in the blank(s) with the appropriate word(s).