A throat swab is inoculated onto a sheep blood agar and then a rapid direct test for Streptococcus pyogenes is performed. The rapid strep test is negative. When streaking the agar plate, you should

A) flame the loop before beginning the final quadrant.
B) flame the loop after completion of all quadrants.
C) stab the agar in the area of the initial specimen placement after completion of the final quadrant.
D) both B and C.
E) all of the above

D