What technology minimizes the problems of a rigid aperture including protein buildup, coincidence, and a single cell being counted twice?
a. Forward scatter
b. Radiofrequency
c. Hydrodynamic focusing
d. Fluorescence
ANS: C
The use of hydrodynamic focusing avoids the pitfalls and potential problems when using a rigid aperture. The outer sheath fluid minimizes protein buildup and plugs, eliminates recirculation of cells, and reduces pulse height irregularity and coincident passage loss.
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