Serum vitamin B12 is highly unstable when exposed to light and can tolerate only a single absorbance reading before breaking down. Therefore, it is not possible to generate a standard curve for B12
When the concentration of B12 must be ascertained in a patient specimen, how should the technician go about doing so?
A) Calculate the concentration of B12 directly from its absorbance in the solution using Beer's Law.
B) Determine the Km and Vmax of B12 and apply those values to Ohm's Law to calculate the concentration of B12.
C) Generate a standard curve using a more stable vitamin of similar chemical structure.
D) Measure transmittance of B12 and create a standard curve using transmittance against concentration.
A
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