Hb A2 and Hb F can be quantified by:
a. Performing a Brilliant cresyl blue stain and counting the number of positive cells
b. Performing high-performance liquid chromatography
c. Performing cellulose acetate hemoglobin electrophoresis
d. Performing citrate agar hemoglobin electrophoresis
ANS: B
High-performance liquid chromatography can separate and quantify Hb A2 and Hb F, which is important to do when working up patients with possible thalassemic syndromes. None of the other methods quantify them.
Health Professions
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