Which of the following would you include when performing a Gram stain? 

A. Cover only a small area of the specimen on the side with the crystal violet stain
B. Allow the stain to sit for no more than 10 seconds before rinsing
C. Cover the entire specimen area with iodine and allow it to remain for 1 minute
D. Rub the slide to hasten drying
E. Apply exactly 3 drops of alcohol or decolorizer after rinsing away the iodine

C. Cover the entire specimen area with iodine and allow it to remain for 1 minute
Apply iodine and allow it to remain on the slide for 1 minute to increase the cell staining.